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LifeSensors/DU6302-25 µL (panel) Di-Ubiquitin K63-2/DU6302-20UL (panel) Di-Ubiquitin K63-2
LifeSensors/DU6302-25 µL (panel) Di-Ubiquitin K63-2/DU6302-20UL (panel) Di-Ubiquitin K63-2
市场价：
¥720.00
美元价：
432.00
产品分类：
其它酶
公司分类：
Other_enzymes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IQFDiUbTechnology
LifeSensors’sdiubiquitinsubstratesrepresentanewclassofsubstratesforthecontinuousfluorescentmeasurementoftrueisopeptidaseactivity.TheC-terminusofwildtypeubiquitinisconjugatedviaanisopeptidebondtolysine48(K48)ofasecondubiquitinmoleculewiththeresultantdiubiquitinforminganinternallyquenchedfluorescentFRETpair(IQF).Eachubiquitinislabeledwithasinglemoleculeofeitherafluorescentreporter(i.e.TAMRA)orahighlyefficientquenchingdye.CleavageoftheIQFDiUbbydeubiquitylasesleadstoseparationofthefluorophorefromquencherandsubsequentincreaseinobservedfluorescence.
LifeSensors’sdiubiquitinsubstratesrepresentanewclassofsubstratesforthecontinuousfluorescentmeasurementoftrueisopeptidaseactivity.TheC-terminusofwildtypeubiquitinisconjugatedviaanisopeptidebondtolysine48(K48)ofasecondubiquitinmoleculewiththeresultantdiubiquitinforminganinternallyquenchedfluorescentFRETpair(IQF).Eachubiquitinislabeledwithasinglemoleculeofeitherafluorescentreporter(i.e.TAMRA)orahighlyefficientquenchingdye.CleavageoftheIQFDiUbbydeubiquitylasesleadstoseparationofthefluorophorefromquencherandsubsequentincreaseinobservedfluorescence.
IQFDiUbVariants
ThereisaremarkabledifferencebetweenK48-andK63-linkedubiquitinmoleculesintheirthree-dimensionalstructure,whichaccountsfortheirindividualfunctions.Beyonddifferentiallinkages(e.g.K48,K63,K11),LifeSensorshascreatedsubpanelsofIQFdiubiquitinsubstrateswithineachlinkage. BecauseeachDUBislikelytorecognizeandcleavesubstrateswithuniquestericconsiderations,thesesubpanelsvaryinlocationofreporterfluorophoreandquencher. WerecommendthateachDUBbeempiricallyevaluatedagainstapanelofIQFDiUbsubstratestoselectthetheoptimalfluorophore/quencherpairing.
ThereisaremarkabledifferencebetweenK48-andK63-linkedubiquitinmoleculesintheirthree-dimensionalstructure,whichaccountsfortheirindividualfunctions.Beyonddifferentiallinkages(e.g.K48,K63,K11),LifeSensorshascreatedsubpanelsofIQFdiubiquitinsubstrateswithineachlinkage. BecauseeachDUBislikelytorecognizeandcleavesubstrateswithuniquestericconsiderations,thesesubpanelsvaryinlocationofreporterfluorophoreandquencher. WerecommendthateachDUBbeempiricallyevaluatedagainstapanelofIQFDiUbsubstratestoselectthetheoptimalfluorophore/quencherpairing.
IQFDiUbsubstratepanels
InordertoefficientlydeterminetheoptimalsubstrateforanyDUB,orrapidlyassessapanelofvariousDUBs,LifeSensorsofferscomprehensivepanelsforeitherK48 (DiUb48panelcatno.DU0101) orK63linkeddiubiquitins (DiUb63PanelDU0102).Eachpanel containallsixvariantsoftherespectivelinkagealongwithacontrolenzyme(USP2core).Inadditionthereisalsoafull(K48/K63) panel (DU0201) thatcontainsall12variants.
Needbulkquantities?Wewouldbehappytoworkoutacustompricethatfitsyourbudget.Pleaseemail info@lifesensors.com formoreinformation.
ThisproductiscoveredbyUSpatent#8,518,660. Bypurchasingthisproduct,thepurchaseragreestocomplywiththetermsofour LimitedUseLabelLicense.
Info
| Amount | 25µL |
| State | Liquid |
| Concentration | 20µM |
| Buffer | 50mMSodiumMES,pH6.0 |
| Variant | 2 |
| Linkage | K63 |
| Molecularweight | 18,548.4+/-18Da |
| Fluorescentreadout | TAMRA |
| Storage | IQFDiUbsarestablefor4-6monthsat4ºC.Longterm(>6months)storageat-80ºCisrecommended.Avoidrepeatedfreeze/thawcycles. |
Applications
- Kineticcharacterizationofdeubiquitylases(DUBs)
- DemonstrationofnovelDUBactivityand/orlinkagespecificity
- MonitoringofheterologousDUBproduction/purification
- Highthroughputscreening(HTS)forantagoNISTsoragonistsof deubiquitylaseactivity
- Additionalmaterialsrequired(forfurtherdetails,pleaseseemanual):
- 384-(GreinerBioOne781209) or96-well(655076)blackplatesorequivalent
- Fluorescentplatereader
- FiltersormonochromatorsformonitoringfluorescenceofTAMRA(Ex540nm,Em580nm)
- Dichroicmirrorwitha550-570nmcutoffisrecommendedforoptimalsignal-to-background
Benefits
- MoreBIOLOGicallyrelevantsubstratecomparedtotrADItionalmono-ubiquitinderivatives.
- FluorophoresareNOTattheN-orC-terminus,modificationsknowntoperturbubiquitinstructure.
- AvarietyoflinkagesandfluorophorepositioningavailableforoptimalactivityofyourDUBofinterest.
- Validatedinboth96-welland384-wellformat.
Documents
品牌介绍
LifeSensors公司目前主要的产品有400多种，其产品主要覆盖泛素和泛素蛋白酶体通路等科学应用领域，并且产品线在不断的扩大中。公司开创了两大独家技术平台：用于细胞信号和药物发现的泛素蛋白酶体系统(UPS)，以及增强目的蛋白在大肠杆菌、酵母、昆虫和哺乳动物细胞中表达与纯化的SUMO融合表达系统。 泛素蛋白酶体系统正在成为继激酶之后，药物发现的下一个机遇，必将成为未来许多新型药物的创新来源。作为泛素蛋白领域的先驱公司，药物的发现、分析化合物以及去泛素酶和泛素化酶的高通量筛选正是LifeSensors创新技术的真正亮点。此外，LifeSensors还为泛素的蛋白质组学提供众多定制技术服务，并且已经开发了各种各样的测定细胞中的生理底物或生物标志物的方法。
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