LifeSensors/UM411B UbiTest Buffers Only UbiTest Buffers Only UbiTest Buffers/UM411B UbiTest Buffers Only UbiTest Buffers Only UbiTest Buffers-免疫在线蚂蚁淘旗下平台

当前位置：首页 > 产品中心 > Other_enzymes > LifeSensors/UM411B UbiTest Buffers Only UbiTest Buffers Only UbiTest Buffers/UM411B UbiTest Buffers Only UbiTest Buffers Only UbiTest Buffers

商品详细LifeSensors/UM411B UbiTest Buffers Only UbiTest Buffers Only UbiTest Buffers/UM411B UbiTest Buffers Only UbiTest Buffers Only UbiTest Buffers

LifeSensors/UM411B UbiTest Buffers Only UbiTest Buffers Only UbiTest Buffers/UM411B UbiTest Buffers Only UbiTest Buffers Only UbiTest Buffers

商品编号： UM411B-UbiTestBuffersOnlyUbiTestBuffersOnly

品牌： LifeSensors

市场价： ¥2200.00

美元价： 1320.00

产地：美国(厂家直采)

公司：

产品分类：其它酶

公司分类： Other_enzymes

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Thiskitallowsyoutodeterminewhetherornotyourtargetproteinisubiquitylated.

Themostcommonmethodtoinvestigateproteinubiquitylationisthroughimmunoprecipitationandimmunoblotanalysis;however,thesubstrateantibodymayinteractdifferentlywithaubiquitylatedsubstrateduetoepitopemasking,reducedaffinity,orchangesinselectivity.Amoredefinitivemethodfordemonstratingproteinubiquitylationistocoupleimmunoprecipitationwithdigestionbyabroadspectrumdeubiquitylasepriortoimmunoblotanalysis.Anincreasedsignalfortheproteinofinterest(POI)afterDUBtreatmentisaclearindicationthattheproteinwasubiquitylatedeveniftherewasnoclearreactivityintheuntreatedsample.ToavoidpotentialproblemsarisingfromchangesinimmunoreactivityofthePOI,itisbesttopull-downtheproteinwithananti-ubiquitinreagent.WehavebuiltthiskitaroundtheuseofTandemUbiquitinBindingEntities(TUBEs),whichareengineeredtandemUBAswithdissociationconstantsfortetra-ubiquitininthenanomolarrange.ThenanomolaraffinityofTUBEsforpolyubiquitinatedproteinsallowshighefficiencyisolationandcharacterizationoftheseproteinsfromcelllinesandtissues.TUBE1hasbeendemonstratedtobindtoall7linkagetypes.

TUBE1isbasedonUBAsfromtheproteinubiquilin.AgarosecoupledTUBEsallowefficientrecoveryofpolyubiquitinatedproteinsinasinglestep.PolyubiquitinatedproteincaneasilybeseparatedfromTUBEsforproteomicstudiesoridentificationbyWesternblotting.

ThisproductisprotectedbyoneormoreUSorForeignpatents.PleasereadtheLimitedUseLabelLicensetolearnmore.Bypurchasingthisproduct,thepurchaseragreestocomplytotheseterms.

品牌介绍

LifeSensors公司目前主要的产品有400多种，其产品主要覆盖泛素和泛素蛋白酶体通路等科学应用领域，并且产品线在不断的扩大中。公司开创了两大独家技术平台：用于细胞信号和药物发现的泛素蛋白酶体系统(UPS)，以及增强目的蛋白在大肠杆菌、酵母、昆虫和哺乳动物细胞中表达与纯化的SUMO融合表达系统。泛素蛋白酶体系统正在成为继激酶之后，药物发现的下一个机遇，必将成为未来许多新型药物的创新来源。作为泛素蛋白领域的先驱公司，药物的发现、分析化合物以及去泛素酶和泛素化酶的高通量筛选正是LifeSensors创新技术的真正亮点。此外，LifeSensors还为泛素的蛋白质组学提供众多定制技术服务，并且已经开发了各种各样的测定细胞中的生理底物或生物标志物的方法。

公司介绍

品牌分类

Autophagy SUMO Protein Expression Systems 工具箱和盘子试管：泛素亲和矩阵 PROTAC® Ubiquitin Chains and Derivatives 抗体和抑制剂泛素酶

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.lifesensors.com/