Specifications
Todate,detectingchangesintheubiquitylationofspecificsubstrateproteinsinresponsetoexternalstimuli,e.g.stress,cytokineexposure,drugcandidatetreatment,etc.,hasbeenalong,labor-intensiveprocessinvolvingimmunoprecipitationfollowedbygelelectrophoresisandWesternblotanalysis. Thismethodislowthrough-put,resourceintensive,andonlysemi-quantitativeatbest. WehavedevelopedtheUbiQuant™Skitasafacile,robust,andquantitativealternativetoIP/WBanalysis.BuiltonLifeSensorsUbiQuantplatform,thesystemcapturesubiquitylatedproteinsinthewellsofamicrotiterplateandthenusesanantibodytotheproteinofinterest(eithernativeortagged)toquantitatetheamountoftheproteinbound.
Note:Ifusingatagged-substrate,theusermustspecifytheepitopetaginordertoreceivetheappropriateantibodyfordetection(e.g.myc,HA,FLAG®,V5,GST,etc. Unfortunately,His6cannotbeusedforthisassay.)
